The MSF and Mitochondrial Targeting
Earth History Research Center

1.The protein now enters the matrix. It is immediately associated with a matrix chaperone, preventing its premature folding.

2. After entering the cytoplasm, the signal portion of the leader is excised and discarded, and the protein commences folding.

3. Either with or without the help of chaperones, the protein achieves its active conformation and begins catalysis.