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5. The second two membrane passages of the protein now move out of the translocon into the membrane itself, as the protein growth continues. Subsequent synthesis of the peptide again continues to accumulate outside of the membrane until another Signal-Anchor Sequence and Stop-Transfer Membrane-Anchor Sequence have been completed.
6. This third set of membrane-soluble helices are then inserted into the Translocon.
7, 8. The procedure is repeated until all 24 membrane crossings are completed. The amino and carboxyl ends of the protein remain in the cytoplasm.